Molecular Biology
TaqMan实时RT-PCR
TaqMan real-time RT-PCRA quantitative PCR method using a hydrolysis probe (TaqMan probe) for the simultaneous detection of multiple targets. / 一种使用水解探针（TaqMan探针）的定量PCR方法，用于同时检测多个靶标。
- Knowledge type
- 工艺技术
- First proposed or discovered
- Proposer
- Turkey Farm member
- Creator
- 农场主
- Last updated
- 2026-10-23 09:01:54
Sub-knowledge points
Multiplexing capabilityReal-time quantificationHigh specificity
Higher-order knowledge points
Nucleic Acid AmplificationMolecular Diagnostics
Main parameters and performance indicators
Probe: Hydrolysis probe labeled with a fluorescent reporter and quencherEnzyme: DNA polymerase with 5' to 3' exonuclease activity (Taq)Template: RNA (reverse transcribed to cDNA)
Open problems
No unresolved problem recorded yet
Related literature
ranked by community starsThe use of double-tube multiplex TaqMan real-time RT-PCR for the simultaneous detection of foodborne viruses in shellfishOpen source
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Creation and modification trail
1 recordsRecord title: TaqMan real-time RT-PCR.
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