Analytical Chemistry

离心微流控芯片生物传感器

Centrifugal Microfluidic Chip Biosensor

A lab-free biosensor platform based on centrifugal microfluidics for on-site multiplex detection of meat adulteration. It integrates immuno-proximity ligation assay (PLA) with nicking enzyme-assisted rolling circle amplification (RCA) to convert protein detection into nucleic acid signal amplification, utilizing G-quadruplex DNAzymes for colorimetric detection and berberine-binding aptamers for fluorescence enhancement. / 一种基于离心微流控技术的无实验室生物传感器平台，用于现场多肉类掺假检测。它整合了免疫邻近连接测定（PLA）与限制性核酸内切酶辅助的滚环扩增（RCA），将蛋白质检测转化为核酸信号扩增，利用G-四链体DNA酶进行比色检测，并结合小檗碱结合适体进行荧光增强。

Knowledge type: 物理设备
First proposed or discovered
Proposer: Turkey Farm member
Creator: 农场主
Last updated: 2026-10-28 21:02:01

Sub-knowledge points

Immuno-proximity ligation assay (PLA)Nicking enzyme-assisted rolling circle amplification (RCA)G-quadruplex DNAzyme generationBerberine-binding aptamer fragments

Higher-order knowledge points

Multiplex meat adulteration monitoringOn-site food authenticationNucleic acid signal amplification-assisted protein diagnostics

Main parameters and performance indicators

Detection limit: 0.056 ng/mL (colorimetric), 0.013 ng/mL (fluorescent)Analysis time: 90 minAdulteration detection limit: 0.5% (w/w)Recovery rate: 95.0%−108.9%

Open problems

No unresolved problem recorded yet

Related literature

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Engineering a Lab-Free Microfluidic Chip Biosensor Integrating Immuno-Proximity Ligation and On-Cleavage RCA for Multiplex Meat Adulteration MonitoringOpen source

离心微流控芯片生物传感器

Sub-knowledge points

Higher-order knowledge points

Main parameters and performance indicators

Open problems

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