Genomics
染色质免疫共沉淀测序
ChIP-seqChromatin immunoprecipitation followed by sequencing (ChIP-seq) is a high-throughput method used to identify protein-DNA interactions and map the genomic locations of DNA-binding proteins. / 染色质免疫共沉淀测序是一种高通量方法，用于鉴定蛋白质-DNA相互作用并绘制DNA结合蛋白的基因组定位。
- Knowledge type
- 工艺技术
- First proposed or discovered
- Proposer
- Turkey Farm member
- Creator
- 农场主
- Last updated
- 2026-11-01 17:05:59
Sub-knowledge points
Nuclei isolation via sucrose gradientCENP-A chromatin immunoprecipitationCENP-C chromatin immunoprecipitationDNA extraction and purificationLibrary preparation and size selectionqPCR to assess enrichment
Higher-order knowledge points
Chromosome organizationCentromere functionGenome stability
Main parameters and performance indicators
Chromatin fragmentation: Micrococcal nuclease (MNase) digestion or sonicationImmunoprecipitation: Protein G Sepharose beadsLibrary preparation: Size selection and adapter ligationData analysis: qPCR to assess enrichment
Open problems
Low signal-to-noise ratios in repetitive regionsLimited reproducibility in centromeric regions
Related literature
ranked by community starsOptimized Genome‐Wide Profiling of Centromeric Chromatin Complexes by ChIP‐seqOpen source
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Creation and modification trail
1 recordsRecord title: ChIP-seq.
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