Analytical Chemistry

亲水相互作用液相色谱

Hydrophilic Interaction Liquid Chromatography

A chromatographic separation technique based on the partitioning of analytes between a hydrophilic stationary phase and an aqueous-organic mobile phase. / 一种基于亲水固定相与水-有机流动相之间分析物分配的色谱分离技术。

Knowledge type: 工艺技术
First proposed or discovered
Proposer: Turkey Farm member
Creator: 农场主
Last updated: 2026-11-11 21:07:46

Sub-knowledge points

B-PEI-0.01 adsorbentB-PEI-1.0 adsorbentTanaka HILIC testRetention timeSelectivityPeak symmetry

Higher-order knowledge points

Microfluidics and Lab on Chip / 工艺技术Chromatography

Main parameters and performance indicators

Stationary phase: Silica-based with covalently bonded polyethyleneimine (PEI)Mobile phase: Aqueous-organic mixtureSeparation mechanism: Partitioning and adsorption

Open problems

Uneven distribution of the PEI functional layer on the substrate surfacePeak symmetry deterioration for amino acids at high PEI content

Related literature

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Silica-based stationary phases with varied content of covalently bonded polyethyleneimine layer for hydrophilic interaction liquid chromatographyOpen source

亲水相互作用液相色谱

Sub-knowledge points

Higher-order knowledge points

Main parameters and performance indicators

Open problems

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